RESEARCH EXPERIENCE

• Post-doctoral Fellow, Department of Veterinary Microbiology and Preventive
  Medicine, Iowa State University, Ames, Iowa (Nov. 2004 - Feb. 2007)
  Supervisor: Dr. Lisa K. Nolan

• Post-doctoral Fellow, Department of Pathobiology, University of Guelph, Canada (Sept. 2002 - Aug. 2003)
  Supervisor: Dr. Shayan Sharif

ACADEMIC EXPERIENCE

• Senior Lecturer, Department of Farm Animal Production and Health, Faculty of
  Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka, Aug.
  2003 to Nov. 2004

• Member, Panel of Teachers/Examiners of the Postgraduate Institute of Science, University of Peradeniya, Sri Lanka, from Nov., 2003 to Nov. 2004

• Lecturer, Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka, June 1992 to Aug. 2003 (on study leave from Sept. 1998 to Aug. 2003).

• Clinician, Veterinary Teaching Hospital, University of Peradeniya, Sri Lanka,
  June 1992 to Aug. 1998

MEMBERSHIPS IN PROFESSIONAL ORGANIZATIONS

• American Society for Microbiology

• American Association of Veterinary Laboratory Diagnosticians

• American Association of Avian Pathologists - member of the AAAP enteric disease committee and emerging diseases committee

• Conference of Research Workers in Animal Disease

• American College of Veterinary Microbiologists

PUBLICATIONS IN REFEREED JOURNALS

Johnson, T.J., Kariyawasam, S., Wannemuehler, Y., Mangiamele, P., Johnson, S.J., Doetkott, C., Skyberg, J.A., Lynne, A.M., Nolan, L.K. Genome sequence of avian pathogenic Escherichia coli strain O1:K1 shares strong similarities with human EXPEC genomes. J. Bacteriol. (Submitted in Nov., 2006)

*S. Kariyawasam *and L.K. Nolan. Common and specific virulence-associated markers of avian and human extraintestinal pathogenic Escherichia coli as determined by genomic subtractive hybridization. Microbiol. (Submitted in Nov., 2006).

S. Kariyawasam, *T.J. Johnson, and L.K. Nolan. 2006. Occurrence of pathogenicity island 1~APEC-O1~ genes among Escherichia coli implicated in avian colibacillosis. Avian Dis. *50:405-410.

S. Kariyawasam, *T.J. Johnson, and L.K. Nolan. 2006. Unique DNA sequences of avian pathogenic Escherichia coli isolates as determined by genomic suppression subtractive hybridization. FEMS Microbiol. Lett. *262:193-200.

S. Kariyawasam, *T.J. Johnson, and L.K. Nolan. 2005. pap operon of avian pathogenic _Escherichia coli _strain O1 is located on a novel pathogenicity island. Infect. Immun. *74:744-749.* *

M.D. Lambourne, W. Si, P.K. Niemiec, L. Read, S. Kariyawasam, *and S. Sharif. 2005. Identification of novel polymorphisms in the B-LB locus of Gallus lafayettei. Animal Genet. *36:435-462.

S. Kariyawasam, B.N. Wilkie, and C.L. Gyles. 2004. Resistance to Escherichia coli respiratory tract infection induced by passively transferred egg-yolk antibodies. Vet. Microbiol. 98:273-284.

S. Kariyawasam, B.N. Wilkie, and C.L. Gyles. 2004. Construction, characterization and evaluation of the vaccine potential of three genetically defined mutants of avian pathogenic Escherichia coli. Avian Dis. 48:287-299.

S. Kariyawasam, B.N. Wilkie, D.B. Hunter, and C.L. Gyles. 2002. Systemic and mucosal immune responses to selected cell surface antigens of avian pathogenic Escherichia coli in experimentally infected chickens. Avian Dis. 46:668-678.

Indira Silva and S. Kariyawasam.* 1996. Post-phagocytic bactericidal activity of buffalo (Bubalus bubalis) neutrophils against mastitis causing bacteria. J. Natnl. Sci. Coun. Sri Lanka. *24 (2):71-80.

PUBLICATIONS IN THE PROCEEDINGS OF INTERNATIONAL CONFERENCES

S. Kariyawasam, T. J. Johnson, and L.K. Nolan. Extensive Analysis of Pathogenicity Island PAI 1~APEC O1~ of Avian Pathogenic Escherichia coli. American Association of Avian Pathologists, American Veterinary Medical Association. July 2007. Washington D.C. (Proposed).

S. Kariyawasam, and L.K. Nolan. A ?tia mutant of an avian pathogenic Escherichia coli. 56th Western Poultry Disease Conference (WDPC). March, 2007. Las Vegas, Nevada (Accepted).

S. Kariyawasam, T. J. Johnson, and L.K. Nolan. ?ire_A mutant of an avian pathogenic _Escherichia coli is not attenuated. Poster presentation at the Conference of Research Workers of Animal Diseases (CRWAD), Dec. 2006. Chicago, Illinois.

S. Kariyawasam and L.K. Nolan. papA gene of avian pathogenic Escherichia coli. Poster presentation at the International Symposium on Virulence Mechanisms of Bacterial Pathogens, Sept. 2006. Ames, Iowa.

S. Kariyawasam, T. J. Johnson, and L.K. Nolan. A novel virulence marker of avian pathogenic Escherichia coli. Poster presentation at the American Association of Avian Pathologists, American Veterinary Medical Association. July 2006. Honolulu, Hawaii.

S. Kariyawasam and L.K. Nolan. A uropathogenic-specific protein is present in avian Escherichia coli. Poster presentation at the American Society for Microbiology. May 2006. Orlando, Florida.

L.K. Nolan, T.J. Johnson, S. Kariyawasam, J. Skyberg, A .Lynne, Y. Wannemuehler, and J. Scaccianoce. Avian Escherichia coli Virulence and Antimicrobial Resistance. Iowa Egg Industry Symposium, November 2005. Ames, Iowa,

S. Kariyawasam, K. Siek, and L.K. Nolan. Use of subtractive hybridization to detect genomic differences between avian pathogenic and non-pathogenic Escherichia coli. Poster presentation at the American Association of Avian Pathologists, American Veterinary Medical Association, July 2005. Minneapolis, Minnesota.

C. L. Gyles, S. Kariyawasam, and B.N. Wilkie. Alternative approaches to control_ Escherichia coli_ infections in chickens. Oral presentation at the International Conference on Veterinary Vaccines, July 2003. University of Guelph, Guelph, Canada.

S. Kariyawasam, B.N. Wilkie, and C.L. Gyles. Passive immunization of chickens against_ Escherichia coli_ respiratory tract infection. Poster presentation at the Conference of Research Workers of Animal Diseases (CRWAD), Nov. 2002. Missouri, USA.

S. Kariyawasam, B.N. Wilkie, and C.L. Gyles. Immune responses to selected cell surface antigens of avian pathogenic Escherichia coli in experimentally infected chickens. Oral presentation at the Conference of Research Workers of Animal Diseases (CRWAD), Nov. 2001. Missouri, USA.

CURRENT RESEARCH SUPPORT

• Principal investigator: A live vaccine against avian colibacillosis. US Poultry and Egg Association. 2006 -2007.

• Principal investigator: A vaccine to protect chickens from Escherichia coli respiratory
  tract infection, United States Department of Agriculture (USDA) Formula Funds Grant Program. 2006.

• Co-investigator: Recombinant Iss protein as a vaccine against avian colibacillosis. Iowa Livestock Health Advisory Council. 2006.